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KMID : 1234420100380020136
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 2 p.136 ~ p.143
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Detection of Novel Polyketide Synthase Genes in Sorangium cellulosum Isolated in Korea
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Youn Jin-Kwon
Kim Do-Hee
Lee Han-Bit
Lee Kye-Won
Cho Kung-Yun
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Abstract
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DNA fragments encoding the ketosynthase (KS) domain of polyketide synthase (PKS) genes were amplified using polymerase chain reaction (PCR) from 9 strains of Sorangium cellulosum isolated in Korea, cloned into a plasmid vector and sequenced. A total of 83 cloned DNA fragments were analyzed, and similar fragments were excluded, leaving 43 independent DNA fragments encoding the KS domains. The predicted amino acid sequences of 32 fragments were 70%-100% identical to the amino acid sequences of already known PKS genes, while the remaining 11 fragments were ≤67% or less identical to the known sequences, suggesting that these genes are novel PKS genes.
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KEYWORD
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Myxobacteria, polyketide synthase, PKS, Sorangium cellulosum
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